Srivatsan Raman

Assistant Professor of Biochemistry

441B HF DeLuca Biochemistry Laboratories sraman4@wisc.edu, 608.890.1036

Departments

Biochemistry

 

Education

Ph.D (2010), University of Washington, Seattle

Postdoctoral fellowship (2015), Harvard Medical School, Boston, MA

Research Interests

Our laboratory takes a systems and synthetic biology approach to understanding and designing biology at multiple scales:   protein, gene regulation, metabolic pathways and, bacteriophages.

Lab Website

https://ramanlaboratory.org

Representative Awards

  • 2018 NIH New Innovator Award
  • 2016 Shaw Scientist Award

Research

Our laboratory takes a systems and synthetic biology approach to understanding and designing biology at multiple scales: proteins, gene regulation, metabolic pathways, and bacteriophages. We are interested in understanding the molecular basis of protein allostery, designing allosteric small molecule biosensors, understanding fundamental principles of bacterial transcription regulation and designing new transcriptional systems, and designing bacteriophages with new host specificities and regulation. To address these questions, we leverage computational protein design (Rosetta), next-generation DNA synthesis and sequencing, and highly multiplexed selection and screening assays. We apply machine learning principles and structural modeling on these large datasets to elucidate underlying relationships between sequence, structure and function and to improve design of new function.

Representative Publications  (Google Scholar | PUBMED)

  • Engineered bacteriophages as programmable biocontrol agents
    Huss P, Raman S
    Current Opinion in Biotechnology, 2019, 61, 116-121
  • Design of a transcriptional biosensor for the portable, on-demand detection of cyanuric acid
    Liu X, Silverman AD, Alam KK, Iverson E, Lucks JB, Jewett MC, Raman S
    ACS Synthetic Biology (accepted)
  • De novo design of programmable inducible promoters
    Liu X, Gupta STP, Bhimsaria D, Reed JL, Rodriguez-Martinez JA, Ansari AZ, Raman S
    Nucleic Acids Research, 2019, 47, 10452-10463
  • A regulatory NADH/NAD+ redox biosensor for bacteria.
    Liu Y, Landick R, Raman S
    ACS Synthetic Biology, 2019, 8, 264-273